[PDF] Characterization of a highly specific NQO1-activated near-infrared fluorescent probe and its application for in vivo tumor imaging | Semantic Scholar (2024)

Human NAD(P)H: Quinone Oxidoreductase 1-mediated activation in A549 lung cancer cells containing high levels of hNQO1 induces a dose-dependent photo-cytotoxic response after irradiation, which is demonstrated to enhance the cancer selectivity of photodynamic therapy.

In this review, the recent progress of NQO1 probes based on organic small molecules is summarized from the perspectives of molecular design strategies, sensing mechanisms and bioimaging applications to further promote the development of versatile imaging tools for NZO1-related biomedical investigation.

A markedly high enzyme level was observed in a xenograft tumor tissue compared with that in a normal tissue, which suggests that such an NQO1 probe is promising for cancer diagnosis and for studying the enzyme-associated biology.

The incorporation of biomarkers to identify hypoxia is a prerequisite for successful HAP therapies, however, it is equally important to assess the level of specific oxidoreductases required for their activation.

This mini-review summarizes quinone-based fluorescent, near-infrared and two-photon fluorescent probes, as well as nanosystems for delivering the NAD(P)H:quinone oxidoreductase 1-activating drug β -lapachone and provides valuable information for the future development of probes and nano-delivery systems that target NAD(P)H:quinone oxidoreductase 1.

This review systematically focused on the recent progress of small molecular near‐infrared fluorescent probes and nano‐fluorescent probes as new biomedical imaging tools in the past 3–5 years and it covers the application of tumor biomarker sensing, tumor microenvironment imaging, and tumor vascular imaging, intraoperative guidance and as an integrated platform for diagnosis.

A facile but general way for hNQO1 detection and associated drug screening as well by ECL sensing of the metabolic H2O2 enabled by futile redox cycle reaction is reported.

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NQ-DCP, a new dicyanoisophorone (DCP) based fluorescent probe capable of monitoring hNQO1 activity in vitro and in vivo in both ratiometric and turn-on model, has successfully employed to monitor non-invasive endogenous hNZQO 1 activity in brain tumor cells in vivo and in xenografted tumors developed in nude mice.

A quinone-trigger-based, near-infrared probe whose fluorescence is effectively turned on several hundred-fold through highly selective reduction of the quin one trigger group by hNQO1, with unprecedented, catalytically efficient formation of a fluorescent reporter is disclosed.

In vivo fluorescence imaging specifically detected GPU-167 in tumors 24 h after administration and ex vivo analysis revealed that fluorescence showed a strong correlation with hypoxia inducible factor (HIF)-1 active hypoxic regions, suggesting thatGPU-167 is a promising in vivo optical imaging probe for tumor hypoxIA.

It is shown it is possible to determine hNQO1 presence in previously studied colorectal cancer cells and the unexplored ovarian cancer cell line NIH:OVCAR-3, with respective PNR values of 926 and 34 being obtained.

The work provides a functional probe for tumor hypoxia, and the results suggest the fluorescent response of the probe is due to a decrease in O2 concentration, and not NTR concentration.

A cloaked fluorophore composed of a reporter whose fluorescence is efficiently quenched by it being bound to an activatable trigger group through a novel self-immolative linker, which triggers its triggered turning on in the presence of an up-regulated enzyme in human cancer cells.

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